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Literature summary extracted from

  • Wang, Z.Y.; Brzeska, H.; Baines, I.C.; Korn, E.D.
    Properties of Acanthamoeba myosin I heavy chain kinase bound to phospholipid vesicles (1995), J. Biol. Chem., 270, 27969-27976.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.7 acidic phospholipids enhance the activation of the enzyme by autophosphorylation Acanthamoeba castellanii
2.7.11.7 additional information enzyme is stimulated by its autophosphorylation Acanthamoeba castellanii
2.7.11.7 phosphatidylserine
-
 Acanthamoeba castellanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.7 0.0002
-
 myosin IB soluble, unphosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii
2.7.11.7 0.0003
-
 myosin IA soluble, unphosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii
2.7.11.7 0.0005
-
 myosin IB phospholipid-bound, unphosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii
2.7.11.7 0.0007
-
 myosin IA phospholipid-bound, unphosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii
2.7.11.7 0.0023
-
 myosin IA soluble, phosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii
2.7.11.7 0.0055
-
 myosin IB soluble, phosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii
2.7.11.7 0.064
-
 GRGRSSVYS phospholipid-bound, unphosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii
2.7.11.7 0.064
-
 GRGRSSVYS soluble, phosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii
2.7.11.7 0.11
-
 GRGRSSVYS soluble, unphosphorylated enzyme, pH 7.0, 30°C Acanthamoeba castellanii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.7 membrane binding to the plasma membrane enhances the activity of unphosphorylated enzyme 20fold, substrate myosin I Acanthamoeba castellanii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.7 Mg2+
-
 Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.7 ATP + myosin I heavy chain Acanthamoeba castellanii
-
 ADP + myosin I heavy chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.7 Acanthamoeba castellanii
-
 myosin I heavy chain kinase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.7 ATP + GRGRSSVYS
-
 Acanthamoeba castellanii ADP + GRGRSS(-phosphate)VYS
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase intermolecular autophosphorylation with vesicle-bound enzyme in absence of substrates Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain
-
 Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain higher activity with membrane-bound substrate myosin I Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin I heavy chain substrates are heavy chains of myosin IA and IB Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
2.7.11.7 ATP + myosin IA
-
 Acanthamoeba castellanii ADP + myosin IA phosphate
-
 ?
2.7.11.7 ATP + myosin IB
-
 Acanthamoeba castellanii ADP + myosin IB phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.7 MHCK
-
 Acanthamoeba castellanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.7 30
-
 assay at Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.7 7
-
 assay at Acanthamoeba castellanii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.7 ATP
-
 Acanthamoeba castellanii